CELL BIOLOGY

1. Apoptosis
1.1 Induced apoptosis using TBT
1.2 Visualization of apoptosis using AnnexinV-FITC + PI kit
1.3 Visualization of chromatin condensation using DAPI
1.4 Mitochondria polarization state monitoring using JC-1
1.5 Sub-G1 peak identification

2. Cell culture
2.1 Cell preparation & isolation
- Subculturing monolayer cell cultures
- Peripheral lymphocyte harvesting
2.2 Cell preservation
- Cryogenic preservation and storage of animal cells
- Freezing and thawing cultured cells
2.3 Primary culture
- Pancreatic islets
- Thyrocytes
- Chondrocytes
- Neuron ganglia
2.4 Cytotoxity and depending processes
- Lipid peroxidation
- Dye excluding tests (MTT, neutral red)

3. Signal transduction
3.1 Cytosolic free calcium monitoring
3.2 Cytosolic hydrogen peroxide monitoring
3.3 Cytosolic nitric oxide monitoring

MOLECULAR BIOLOGY

4. Cloning
4.1 PCR product cloning
4.2 Vector preparation

5. DNA
5.1 DNA extraction & purification
- DNA extraction from acrylamide gel
- DNA extraction from agarose gel
- DNA extraction from blood
- DNA extraction from cell and tissue
- DNA extraction from paraffin section

6. Electrophoresis
6.1 Agarose electrophoresis
6.2 Polyacrylamide gel electrophoresis (PAGE)

7. PCR
7.1 PCR product purification
7.2 Real-time PCR
7.3 RT-PCR
7.4 Standard PCR

8. RNA
8.1 cDNA
8.2 Reverse transcription (RT)
8.3 RNA electrophoresis

9. Transfection
9.1 Electroporation
9.2 Stable transfection
9.3 Transfection control using GFP

10. Protein
10.1 Extraction and purification
10.2 Western blotting